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nebnext multiplex oligos  (New England Biolabs)


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    New England Biolabs nebnext multiplex oligos
    Comparison of MECP2 wt and HM maps with MeDIP and hMeDIP maps. ( a ) EMSA analysis of monoclonal anti-hmC antibodies (1:10 dilution) binding to dsDNA <t>oligos</t> (0.75 pM) bearing a single hmC/mC dyad (for data with hmC-modified ssDNA, see ). HM: MECP2 HM positive control, EG, DG, CS: antibody supplier (see the “Materials and methods” section). ( b ) EMSA analysis of polyclonal anti-hmC antibody binding to dsDNA oligo (0.75 pM) containing the indicated CpG dyad modifications. Antibody dilutions on top. ( c ) Bar diagram of quantification of EMSA data from Fig. , error bars show standard deviation ( n = 2). ( d ) Venn diagram for peaks from MeDIP and MECP2 wt enrichments. ( e ) Enrichment/depletion of CpG islands for selected peak sets. ( f ) Venn diagram for peaks from hMeDIP and MECP2 HM enrichments. ( g, h ) Enrichment/depletion of CpG islands and TTS for selected peak sets.
    Nebnext Multiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA"

    Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkag389

    Comparison of MECP2 wt and HM maps with MeDIP and hMeDIP maps. ( a ) EMSA analysis of monoclonal anti-hmC antibodies (1:10 dilution) binding to dsDNA oligos (0.75 pM) bearing a single hmC/mC dyad (for data with hmC-modified ssDNA, see ). HM: MECP2 HM positive control, EG, DG, CS: antibody supplier (see the “Materials and methods” section). ( b ) EMSA analysis of polyclonal anti-hmC antibody binding to dsDNA oligo (0.75 pM) containing the indicated CpG dyad modifications. Antibody dilutions on top. ( c ) Bar diagram of quantification of EMSA data from Fig. , error bars show standard deviation ( n = 2). ( d ) Venn diagram for peaks from MeDIP and MECP2 wt enrichments. ( e ) Enrichment/depletion of CpG islands for selected peak sets. ( f ) Venn diagram for peaks from hMeDIP and MECP2 HM enrichments. ( g, h ) Enrichment/depletion of CpG islands and TTS for selected peak sets.
    Figure Legend Snippet: Comparison of MECP2 wt and HM maps with MeDIP and hMeDIP maps. ( a ) EMSA analysis of monoclonal anti-hmC antibodies (1:10 dilution) binding to dsDNA oligos (0.75 pM) bearing a single hmC/mC dyad (for data with hmC-modified ssDNA, see ). HM: MECP2 HM positive control, EG, DG, CS: antibody supplier (see the “Materials and methods” section). ( b ) EMSA analysis of polyclonal anti-hmC antibody binding to dsDNA oligo (0.75 pM) containing the indicated CpG dyad modifications. Antibody dilutions on top. ( c ) Bar diagram of quantification of EMSA data from Fig. , error bars show standard deviation ( n = 2). ( d ) Venn diagram for peaks from MeDIP and MECP2 wt enrichments. ( e ) Enrichment/depletion of CpG islands for selected peak sets. ( f ) Venn diagram for peaks from hMeDIP and MECP2 HM enrichments. ( g, h ) Enrichment/depletion of CpG islands and TTS for selected peak sets.

    Techniques Used: Comparison, Methylated DNA Immunoprecipitation, Binding Assay, Modification, Positive Control, Standard Deviation



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    Comparison of MECP2 wt and HM maps with MeDIP and hMeDIP maps. ( a ) EMSA analysis of monoclonal anti-hmC antibodies (1:10 dilution) binding to dsDNA <t>oligos</t> (0.75 pM) bearing a single hmC/mC dyad (for data with hmC-modified ssDNA, see ). HM: MECP2 HM positive control, EG, DG, CS: antibody supplier (see the “Materials and methods” section). ( b ) EMSA analysis of polyclonal anti-hmC antibody binding to dsDNA oligo (0.75 pM) containing the indicated CpG dyad modifications. Antibody dilutions on top. ( c ) Bar diagram of quantification of EMSA data from Fig. , error bars show standard deviation ( n = 2). ( d ) Venn diagram for peaks from MeDIP and MECP2 wt enrichments. ( e ) Enrichment/depletion of CpG islands for selected peak sets. ( f ) Venn diagram for peaks from hMeDIP and MECP2 HM enrichments. ( g, h ) Enrichment/depletion of CpG islands and TTS for selected peak sets.
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    Image Search Results


    Comparison of MECP2 wt and HM maps with MeDIP and hMeDIP maps. ( a ) EMSA analysis of monoclonal anti-hmC antibodies (1:10 dilution) binding to dsDNA oligos (0.75 pM) bearing a single hmC/mC dyad (for data with hmC-modified ssDNA, see ). HM: MECP2 HM positive control, EG, DG, CS: antibody supplier (see the “Materials and methods” section). ( b ) EMSA analysis of polyclonal anti-hmC antibody binding to dsDNA oligo (0.75 pM) containing the indicated CpG dyad modifications. Antibody dilutions on top. ( c ) Bar diagram of quantification of EMSA data from Fig. , error bars show standard deviation ( n = 2). ( d ) Venn diagram for peaks from MeDIP and MECP2 wt enrichments. ( e ) Enrichment/depletion of CpG islands for selected peak sets. ( f ) Venn diagram for peaks from hMeDIP and MECP2 HM enrichments. ( g, h ) Enrichment/depletion of CpG islands and TTS for selected peak sets.

    Journal: Nucleic Acids Research

    Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA

    doi: 10.1093/nar/gkag389

    Figure Lengend Snippet: Comparison of MECP2 wt and HM maps with MeDIP and hMeDIP maps. ( a ) EMSA analysis of monoclonal anti-hmC antibodies (1:10 dilution) binding to dsDNA oligos (0.75 pM) bearing a single hmC/mC dyad (for data with hmC-modified ssDNA, see ). HM: MECP2 HM positive control, EG, DG, CS: antibody supplier (see the “Materials and methods” section). ( b ) EMSA analysis of polyclonal anti-hmC antibody binding to dsDNA oligo (0.75 pM) containing the indicated CpG dyad modifications. Antibody dilutions on top. ( c ) Bar diagram of quantification of EMSA data from Fig. , error bars show standard deviation ( n = 2). ( d ) Venn diagram for peaks from MeDIP and MECP2 wt enrichments. ( e ) Enrichment/depletion of CpG islands for selected peak sets. ( f ) Venn diagram for peaks from hMeDIP and MECP2 HM enrichments. ( g, h ) Enrichment/depletion of CpG islands and TTS for selected peak sets.

    Article Snippet: Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using NEBNext Multiplex Oligos (NEB, E73359).

    Techniques: Comparison, Methylated DNA Immunoprecipitation, Binding Assay, Modification, Positive Control, Standard Deviation